Adjust pH to 8.0, first with NaOH pellets, then with 1 m NaOH solution. Note that EGTA will not go into solution until pH is Adjust final volume to 0.1 L.
EGTA Solution (100 mm). 1. Add g of EGTA (e.g., Sigma-Aldrich to 50 mL ultrapure water in a plastic beaker. 2. Add 20 mL KOH (make fresh from.
calcium- EGTA solutions has been reported. 3. A procedure for making a calibration standard for calcium ion concentration, with detection accurate to. 10 µM in a.